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KMID : 0380020140290050343
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 5 p.343 ~ p.352
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Antioxidant and Nitric Oxide Inhibitory Activities of Pigments from Chionoecetes japonicas Rathbun
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Park Byung-Ju
Baek Seung-Oh
Song Young-Sun
Seo Young-Wan
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Abstract
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In the present study, antioxidant activities of two crude pigments (acetone and MeOH) and their solvent fractions (n-hexane, 85% aq.MeOH, n-BuOH, and water fractions) from red crab shell were evaluated by measuring 1,1- diphenyl-2-picryl hydrazyl (DPPH), peroxynitrites, and degree of production of reactive oxygen species (ROS) in HT 1080 cells as well as the extent of oxidative damage of genomic DNA purified from HT 1080 cells. From comparative analysis, 85% aq.MeOH fraction showed the strongest scavenging effect on both peroxynitrite in vitro and intracellular ROS in HT 1080 cells. Protective activities of these samples against hydroxyl radical-mediated genomic DNA damage were also investigated. 85% aq.MeOH and n-BuOH fractions significantly inhibited oxidative damage of purified genomic DNA. On the other hand, we investigated their inhibitory effects on nitric oxide (NO) production in lipopolysaccharide (LPS)- stimulated Raw 264.7 cells. All samples significantly reduced NO production. Among the samples, n-hexane and water solvent fractions most effectively inhibited NO.
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KEYWORD
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Chionoecetes japonicas, Pigment, Antioxidant, Reactive oxygen species (ROS)
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